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KMID : 1005220120270030155
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Journal of Embryo Transfer
2012 Volume.27 No. 3 p.155 ~ p.162
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Effect of Low Density Lipoprotein (LDL) on Motility, Viability, Membrane Integrity and Acrosome Integrity of Frozen-thawed Sperm in Korean Jeju Black Bull
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Oh Shin-Ae
Choi Sun-Ho
Ko Min-Hee
Kang Tae-Young
Oh Young-Mi
Chung Yung-Ho
Cho Won-Mo
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Abstract
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This study was designed to determine whether low-density lipoporoteins (LDL) extracted from egg yolk in extender improve the function of Korean Jeju Black Bull semen. The semen was cryopreserved with 5% ethylene glycol (EG) or 7% glycerol (G) extenders containing 10% egg yolk (EY), 4% LDL and 5% EY or 8% LDL. Frozen-thawed sperm were evaluated sperm motility, viability, membrane integrity and acrosome integrity. Post-thawed sperm motility has been significantly higher (p<0.05) in 4% LDL + 5% EY (69.00% ± 4.18; EG and 63.00% ± 9.75; 7% G) than 8% LDL (57.00% ± 5.70; EG and 52.00% ± 4.47;G). Treatment of 4% LDL + 5% EY-EG (66.85% ± 5.06) has been significantly improved sperm viability compared to other treatments except 10% EY - EG. Moreover, in membrane integrity, swollen sperm ratio has been only significantly increased (p<0.05) in 4% LDL + 5% EY - EG (64.65% ± 6.10) among all treatments. In assess to detect acrosome integrity, especially, AR pattern ratio has been significantly decreased (p<0.05) in 4% LDL + 5% EY - EG among all treatments. In sperm viability as time passes, between 4% LDL + 5% EY and 10% EY, there was no significant difference, but 8% LDL was significantly decreased sperm viability in EG (1 and 2 hrs) and G (30 min, 1, 2, 5 and 12 hrs) extender. However, there were no significant differences among all treatments except 8% LDL-G in sperm membrane integrity. 8% LDL-G has been significantly decreased swollen sperm ratio at 5 hrs after thawed. It is concluded from these results that 4% LDL + 5% EY to the freezing extender showed more positive effect on the frozen-thawed spermatozoa in Korean Jeju Black bull.
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KEYWORD
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Korean Jeju Black Bull , cryopreservation , semen , low density lipoprotein , LDL
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